Biological assessment of a new ready-to-use hydraulic sealer
Benetti Francine, Gomes-Filho Joao Eduardo, de Azevedo-Queiroz India Olinta, Carminatti Marina, Conti Leticia Citelli, dos Reis-Prado Alexandre Henrique, de Oliveira Sandra Helena Penha, Ervolino Edilson, Dezan-Junior Eloi, Cintra Luciano Tavares Angelo,
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( Benetti Francine ) - Universidade Federal de Minas Gerais School of Dentistry Department of Restorative Dentistry
( Gomes-Filho Joao Eduardo ) - Sao Paulo State University School of Dentistry Department of Preventive and Restorative Dentistry
( de Azevedo-Queiroz India Olinta ) - Sao Paulo State University School of Dentistry Department of Preventive and Restorative Dentistry
( Carminatti Marina ) - Sao Paulo State University School of Dentistry Department of Preventive and Restorative Dentistry
( Conti Leticia Citelli ) - Sao Paulo State University School of Dentistry Department of Preventive and Restorative Dentistry
( dos Reis-Prado Alexandre Henrique ) - Universidade Federal de Minas Gerais School of Dentistry Department of Restorative Dentistry
( de Oliveira Sandra Helena Penha ) - Sao Paulo State University School of Dentistry Department of Basic Sciences
( Ervolino Edilson ) - Sao Paulo State University School of Dentistry Department of Basic Sciences
( Dezan-Junior Eloi ) - Sao Paulo State University School of Dentistry Department of Preventive and Restorative Dentistry
( Cintra Luciano Tavares Angelo ) - Sao Paulo State University School of Dentistry Department of Preventive and Restorative Dentistry
Abstract
Objectives: This study compared the cytotoxicity, biocompatibility, and tenascin immunolabeling of a new ready-to-use hydraulic sealer (Bio-C Sealer) with MTA-Fillapex and white MTA-Angelus.
Materials and Methods: L929 fibroblasts were cultivated and exposed to undiluted and diluted material extracts. Polyethylene tubes with or without (the control) the materials were implanted into the dorsa of rats. At 7 days and 30 days, the rats were euthanized, and the specimens were prepared for analysis; inflammation and immunolabeling were measured, and statistical analysis was performed (p < 0.05).
Results: MTA-Fillapex exhibited greater cytotoxicity than the other materials at all time points (p < 0.05). The undiluted Bio-C Sealer exhibited greater cytocompatibility at 6 and 48 hours than white MTA-Angelus, with higher cell viability than in the control (p < 0.05). White MTA-Angelus displayed higher cell viability than the control at 24 hours, and the one-half dilution displayed similar results at both 6 and 48 hours (p < 0.05). At 7 days and 30 days, the groups exhibited moderate inflammation with thick fibrous capsules and mild inflammation with thin fibrous capsules, respectively (p > 0.05). At 7 days, moderate to strong immunolabeling was observed (p > 0.05). After 30 days, the control and MTA-Fillapex groups exhibited strong immunolabeling, the white MTA-Angelus group exhibited moderate immunolabeling (p > 0.05), and the Bio-C Sealer group exhibited low-to-moderate immunolabeling, differing significantly from the control (p < 0.05).
Conclusions: Bio-C Sealer and white MTA-Angelus exhibited greater cytocompatibility than MTA-Fillapex; all materials displayed adequate biocompatibility and induced tenascin immunolabeling.
키워드
Biocompatibility; Cytotoxicity; Hydraulic sealer; Mineral trioxide aggregate; Tenascin
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